Our group takes advantage of a multidisciplinary approach to study the colonization of vessels wall by bacterial pathogens: (i) Microbiology, microbiological techniques allow us to study the bacterial factors involved in the process; (ii) Cell biology, in vitro cell culture models combined with video imaging techniques provide dynamic view of the infection; (iii) Vascular biology, latest advances in the field of vascular biology to design in vivo and ex vivo infection models. (iv) Biophysics, to take advantage in particular of the large potential of microfluidics; (v) Analytical chemistry to analyze protein prosttranslational modifications with the latest mass spectrometry techniques.